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Acridine Orange. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry characterize multidrug resistance and measure changes in mitochondrial mass during apoptosis in rat thymocytes. Acridine Orange is a metachromatic dye which can stain DNA RNA and acid glycosaminoglycans. L13159 Acridine Orange. This unique characteristic makes acridine orange useful for cell-cycle studies.
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Size Price Quantity Availability. Acridine Orange AO is a nucleic acid selective metachromatic stain useful for cell cycle determination. It enters into acidic organelles in a pH-dependent manner. DNA intercalated AO fluoresces green 525nm. Acridine orangeis a fluorescent dye which easily traverses the cell membrane. Since it is a cationic dye it also enters acidic compartments such as lysosomes which in low pH conditions will emit orange light.
DNA intercalated AO fluoresces green 525nm.
It enters into acidic organelles in a pH-dependent manner. It enters into acidic organelles in a pH-dependent manner. Acridine orange has also been used as. However at high concentrations it denatures and precipitates both RNA and DNA. A fluorescent dye such as acridine orange absorbs the energy of incoming light. Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA.
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Human cells and tissue material stain a pale green to yellow while bacteria stain a bright orange at a pH of 40. Acridine orange Acridine orange is a fluorescent dye. At low concentrations it intercalates into DNA and precipitates RNA. Acridine Orange buffered at a low pH produced differential staining of bacteria and background material in clinical specimens. It enters into acidic organelles in a pH-dependent manner.
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It has a role as a fluorochrome and a histological dye. Acridine orange has also been used as. Once inside the dye is protonated and becomes by this entrapped in these organelles D3. Acridine orange has also been used as. SDS Certificate of Analysis Product Specification Technical Inquiry Stock No.
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Acridine orange Acridine orange is a fluorescent dye. Synonyms 36-Bisdimethylaminoacridine hydrochloride zinc chloride double salt. Acridine Orange AO is a nucleic acid selective metachromatic stain useful for cell cycle determination. Acridine orange AO a basic dye was synthesized by Benda in 1889 and was produced by Badische Anilin Soda Fabrik. The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells ie bacterial cells and white blood cells.
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Acridine orange a vital stain will intercalate with nucleic acid changing the dyes optical characteristics so that it will fluoresce bright orange under ultraviolet light. Synonyms 36-Bisdimethylaminoacridine hydrochloride zinc chloride double salt. Because of its weak basic property it accumulates in lysosomes which have a low pH inside due to an ATP-dependent proton pump present in their membrane. When the organisms are treated with Acridine Orange it binds with the nucleic acid either DNA or RNA by the electrostatic interactions and fluoresces to emit various colors. Acridine orange is a cell-permeable nucleic acid selective dye that emits green fluorescence when bound to dsDNA at 520 and red fluorescence when bound to ssDNA or RNA at 650 nm.
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Acridine orange Acridine orange is a fluorescent dye. Acridine orange has also been used as. Acridine orange is used in epifluorescence microscopy and flow cytometry. Size Price Quantity Availability. Synonyms 36-Bisdimethylaminoacridine hydrochloride zinc chloride double salt.
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Acridine orangeis a fluorescent dye which easily traverses the cell membrane. A fluorescent dye such as acridine orange absorbs the energy of incoming light. Acridine orange a vital stain will intercalate with nucleic acid changing the dyes optical characteristics so that it will fluoresce bright orange under ultraviolet light. Acridine orange AO a basic dye was synthesized by Benda in 1889 and was produced by Badische Anilin Soda Fabrik. Although AO was available from Dr K.
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In 1980 McCarthy and Senne evaluated the use of Acridine Orange for the detection of microorganisms in blood. Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. Because of its weak basic property it accumulates in lysosomes which have a low pH inside due to an ATP-dependent proton pump present in their membrane. Hollborn. Acridine orange a vital stain will intercalate with nucleic acid changing the dyes optical characteristics so that it will fluoresce bright orange under ultraviolet light.
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The hydrochloride salt is the fluorescent dye acridine orange used for cell cycle determination. RNA electrostatically bound AO fluoresces red 630nm. It enters into acidic organelles in a pH-dependent manner. L13159 Acridine Orange. At low concentrations it intercalates into DNA and precipitates RNA.
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It has a role as a fluorochrome and a histological dye. Size Price Quantity Availability. The compound binds to genetic material and can differentiate between deoxyribonucleic acid DNA and ribonucleic acid RNA. Acridine Orange a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA is used in fluorescence and epiflouresence microscopy. Acridine Orange AO is a slightly cationic lipophilic fluorochrome stain capable of permeating cell and organelle membrane structure.
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Acridine orange is a cell-permeable nucleic acid selective dye that emits green fluorescence when bound to dsDNA at 520 and red fluorescence when bound to ssDNA or RNA at 650 nm. Acridine orange staining uses the rapid identification of Trichomonas vaginalis yeast cells and clue cells in vaginal smears. A fluorescent dye such as acridine orange absorbs the energy of incoming light. When the organisms are treated with Acridine Orange it binds with the nucleic acid either DNA or RNA by the electrostatic interactions and fluoresces to emit various colors. This unique characteristic makes acridine orange useful for cell-cycle studies.
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Since it is a cationic dye it also enters acidic compartments such as lysosomes which in low pH conditions will emit orange light. This unique characteristic makes acridine orange useful for cell-cycle studies. At low concentrations it intercalates into DNA and precipitates RNA. It can also use to detect intracellular gonococci meningococci and other bacteria particularly in blood cultures. Since it is a cationic dye it also enters acidic compartments such as lysosomes which in low pH conditions will emit orange light.
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Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry characterize multidrug resistance and measure changes in mitochondrial mass during apoptosis in rat thymocytes. Acridine Orange buffered at a low pH produced differential staining of bacteria and background material in clinical specimens. Size Price Quantity Availability. However at high concentrations it denatures and precipitates both RNA and DNA. Since it is a cationic dye it also enters acidic compartments such as lysosomes which in low pH conditions will emit orange light.
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Acridine orangeis a fluorescent dye which easily traverses the cell membrane. AO interacts with DNA and RNA by intercalation or electrostatic attraction respectively. Acridine orange has also been used as. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry characterize multidrug resistance and measure changes in mitochondrial mass during apoptosis in rat thymocytes. Acridine Orange a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA is used in fluorescence and epiflouresence microscopy.
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Acridine orange staining uses the rapid identification of Trichomonas vaginalis yeast cells and clue cells in vaginal smears. Because of its weak basic property it accumulates in lysosomes which have a low pH inside due to an ATP-dependent proton pump present in their membrane. Acridine orange is used in epifluorescence microscopy and flow cytometry. Acridine orange is a fluorochrome stain. Acridine Orange Stain is used for the differentiation of cellular organelles.
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The energy of the light passes into the dye molecules. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine Orange is a cell-permeant nucleic acid binding dye that emits green fluorescence when bound to dsDNA and red fluorescence when bound to ssDNA or RNA. The compound binds to genetic material and can differentiate between deoxyribonucleic acid DNA and ribonucleic acid RNA. Acridine orange free base is a member of the class of aminoacridines that is acridine carrying two dimethylamino substituents at positions 3 and 6.
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Acridine Orange a cell-permeable metachromatic fluorescent cationic dye that intercalates DNA and RNA is used in fluorescence and epiflouresence microscopy. This unique characteristic makes acridine orange useful for cell-cycle studies. It has a role as a fluorochrome and a histological dye. This unique characteristic makes acridine orange useful for cell-cycle studies. Acridine orange has also been used as.
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This energy cannot be accommodated by the dye forever and so is released. Acridine orange has also been used as. The compound binds to genetic material and can differentiate between deoxyribonucleic acid DNA and ribonucleic acid RNA. The ability to penetrate the cell membranes of acidic organelles and cationic properties of acridine orange allows the dye to differentiate between various types of cells ie bacterial cells and white blood cells. Acridine orange has also been used as.
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Synonyms 36-Bisdimethylaminoacridine hydrochloride zinc chloride double salt. Acridine orange a vital stain will intercalate with nucleic acid changing the dyes optical characteristics so that it will fluoresce bright orange under ultraviolet light. RNA electrostatically bound AO fluoresces red 630nm. Acridine Orange dye has been used to analyze mitochondria and lysosomal content by flow cytometry characterize multidrug resistance and measure changes in mitochondrial mass during apoptosis in rat thymocytes. It can also use to detect intracellular gonococci meningococci and other bacteria particularly in blood cultures.
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