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Calcein. Calcein-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health. Calcein AM Product Information Material Number. The calcein assay is based on the conversion of the cell permeant non-fluorescnt calcein AM dye to the fluorescent calcein dye by intracellular esterase activity in live cells. Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells.
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This method not only analyses cell membrane integrity but also esterase activity. Calcein is used for the fluorometric determination of calcium and EDTA ethylenediaminetetraacet ic acid titration of calcium. Calcein and Propidium Iodide Assay Protocol. Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells. The hydrolysis of Calcein AM by intracellular esterases. Calcein is a xanthene dye.
Propidium iodide PI is membrane impermeant and therefore does not enter viable cells with intact membranes.
Calcein AM Figure 279 is a P-gp substrate which limits its ability to enter the cells. Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. The proton dissociation constants are reported to be pKa 1 21 pKa 2 29 pKa 3 42 pKa 4 55 pKa 5 108 and pKa 6 117. Calcein is used for the fluorometric determination of calcium and EDTA ethylenediaminetetraacet ic acid titration of calcium. It has a role as a fluorochrome. Calcein Red-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases.
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Calcein Red-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases. Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells. When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. For Research Use Only. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases.
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The Calcein AM Cell Viability Assay provides a simple rapid and accurate method to measure cell viability andor cytotoxicity. Cleaving the AM ester allows the probe to excite and emit at 488nm 520nm respectively. Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. This method not only analyses cell membrane integrity but also esterase activity. The proton dissociation constants are reported to be pKa 1 21 pKa 2 29 pKa 3 42 pKa 4 55 pKa 5 108 and pKa 6 117.
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Calcein AM is rapidly hydrolyzed inside the cells to form Calcein. Calcein Red-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases. Calcein and Propidium Iodide Assay Protocol. Cleaving the AM ester allows the probe to excite and emit at 488nm 520nm respectively. Calcein AM is rapidly hydrolyzed inside the cells to form Calcein.
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Calcein is a polyanionic fluorescein derivative that exhibits fluorescence that is essentially independent of pH between 65 and 12. Calcein is a fluorescent dye and self-quenching probe that used as an indicator of lipid vesicle leakage and a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium. The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm. When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. Calcein is a xanthene dye.
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When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. Calcein Assay is a Predictive Test in Acute Myeloid Leukaemia 309. 1 mg Description BD Pharmingen Calcein AM is used for labeling live cells that can be detected and further analyzed by flow cytometry or fluorescence imaging. Calcein is fluorescent and is readily detected using a fluorescence plate reader. It is slightly soluble in water.
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Calcein is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage and also as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium. Cells were selected on forward scatter-side scatter FS-SS dot plot R1 cells with low fluorescence intensity were excluded on FL1 histogram R2 and dead cells were excluded on the basis of their high PI fluorescence R3. The hydrolysis of Calcein AM by intracellular esterases. Calcein is a fluorescent dye and self-quenching probe that used as an indicator of lipid vesicle leakage and a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium. Calcein AM Figure 279 is a P-gp substrate which limits its ability to enter the cells.
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The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm. Bis N N bis carboxymethylaminomethylfluorescein Fluorescein- bis methyliminodiacetic acid Fluorexon. Calcein is a calcium-dependent fluorescent molecule. The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions.
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Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells. The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm. When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. The calcein assay is based on the conversion of the cell permeant non-fluorescnt calcein AM dye to the fluorescent calcein dye by intracellular esterase activity in live cells. It is slightly soluble in water.
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Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Calcein AM is rapidly hydrolyzed inside the cells to form Calcein. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases. Propidium iodide PI is membrane impermeant and therefore does not enter viable cells with intact membranes. It is slightly soluble in water.
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Bis N N bis carboxymethylaminomethylfluorescein Fluorescein- bis methyliminodiacetic acid Fluorexon. Calcein is a fluorescent metal indicator for calcium detection with EDTA titration. Calcein solution emits a yellowish-green fluorescence in acidic conditions and no fluorescence in basic conditions. Cells were selected on forward scatter-side scatter FS-SS dot plot R1 cells with low fluorescence intensity were excluded on FL1 histogram R2 and dead cells were excluded on the basis of their high PI fluorescence R3. Calcein is a xanthene dye.
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Calcein is a polyanionic fluorescein derivative that exhibits fluorescence that is essentially independent of pH between 65 and 12. This versatile green fluorophore is cell impermeant and is commonly used for cell tracing and in studies of endocytosis and gap junctions. Calcein AM Product Information Material Number. Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells. It is a a non-fluorescent probe cleaved to a fluorescent probe by non-specific intracellular esterases.
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It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. The proton dissociation constants are reported to be pKa 1 21 pKa 2 29 pKa 3 42 pKa 4 55 pKa 5 108 and pKa 6 117. It has a role as a fluorochrome. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm.
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When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. Calcein-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health. When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. This method not only analyses cell membrane integrity but also esterase activity. Calcein Red-AM is a fluorogenic cell-permeant fluorescent probe that indicates cellular health by detecting the activity of nonspecific esterases.
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It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. It has a role as a fluorochrome. Calcein AM Product Information Material Number. Calcein is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage and also as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium. Calcein AM is rapidly hydrolyzed inside the cells to form Calcein.
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The enhanced hydrophobicity of the acetomethoxy AM derivative of Calcein allows this dye to readily enter viable cells. Calcein is a fluorescent metal indicator for calcium detection with EDTA titration. 1 mg Description BD Pharmingen Calcein AM is used for labeling live cells that can be detected and further analyzed by flow cytometry or fluorescence imaging. The enhanced hydrophobicity of the acetomethoxy AM derivative of Calcein allows this dye to readily enter viable cells. This method not only analyses cell membrane integrity but also esterase activity.
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When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. Calcein is used for the fluorometric determination of calcium and EDTA ethylenediaminetetraacet ic acid titration of calcium. It has a role as a fluorochrome. The hydrolysis of Calcein AM by intracellular esterases produces calcein a hydrophilic strongly fluorescent compound that is well-retained in the cell cytoplasm. Calcein AM is a non-fluorescent hydrophobic compound that easily permeates intact live cells.
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It derives from a fluoran. When the acetoxymethyl ester is intact this probe is nonfluorescent until acted upon by nonspecific esterases present within the healthy live cells. This dye is also available as 1 mg of the. Calcein is a fluorescent dye and self-quenching probe that used as an indicator of lipid vesicle leakage and a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium. Calcein is a fluorescent dye and self-quenching probe used as an indicator of lipid vesicle leakage and also as a complexometric indicator for titration of calcium ions with EDTA and for fluorometric determination of calcium.
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Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. Cells were selected on forward scatter-side scatter FS-SS dot plot R1 cells with low fluorescence intensity were excluded on FL1 histogram R2 and dead cells were excluded on the basis of their high PI fluorescence R3. The calcein assay is based on the conversion of the cell permeant non-fluorescnt calcein AM dye to the fluorescent calcein dye by intracellular esterase activity in live cells. It has a role as a fluorochrome. Calcein am is an organic heteropentacyclic compound that is calcein in which all four carboxy group hydrogens have been substituted by acetyloxymethoxy groups and the hyrodgens of the two hydroxy groups have been substituted by acetyl groups.
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