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Cole1 Plasmid. 105310 Plasmid copy number of pMTL500Eres plasmid. ColE1 is the prototype of many closely related high-copy-number plasmids. PWE15 plasmid characteristic copy number ColE1 ori bacteria ID. This plasmid is available through Addgene.
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ColE1 does not require any plasmid-encoded proteins for regulation of its copy number. Host RNA polymerase synthesizes a preprimer RNAII of 550 nt in length. Bacteria Bacillus cereus ID. Here we show that plasmid pBR322 contains a functional bom sequence located within a. ColE1 is the prototype of many closely related high-copy-number plasmids. Bacterial cells harboring each of the mutant plasmids were tested.
Some common ones you might see include ColE1 pMB1 which comes in a few slightly different but well known derivatives pSC101 R6K and 15A.
Hershfield V Boyer HW Yanofsky C Lovett MA Helinski DR. ColE1 is the prototype of many closely related high-copy-number plasmids. ColE1 plasmid replication is tightly controlled by an antisense RNA mechanism that is highly dynamic tuning plasmid metabolic burden to the physiological state of the host. In these cells copy number of plasmids pOri1 and pOri2 was significantly increased indicating that RPI may be a limiting factor for ColE1-like plasmid DNA replication indeed. Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. For all complete IncX1 plasmids there was a 21-base pair bp difference in strains carrying the Col plasmids 37 824 bp compared to strains with no Col plasmids 37.
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This plasmid is available through Addgene. Some common ones you might see include ColE1 pMB1 which comes in a few slightly different but well known derivatives pSC101 R6K and 15A. Plasmid ColE1 as a molecular vehicle for cloning and amplification of DNA. Some will produce many plasmid copies and others produce just a few copies depending on how they are regulated. ColE1 plasmid replication is tightly controlled by an antisense RNA mechanism that is highly dynamic tuning plasmid metabolic burden to the physiological state of the host.
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