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Cryptic Plasmid. Cryptic plasmids are essentially in a commensal relationship with its host - the plasmid benefits by utilizing its reproductive machinery but the host gains no benefit. A new cryptic plasmid named pMM101 in an environmental Pseudomonas sp. A plasmid to which no phenotypic traits have been ascribed. The total probable coding capacity of pJD1 was determined from the complete nucleotide sequence by using computational probes to identify open reading frames with similar codon usage and by screening for the presence of ribosomal binding sites before the start.
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A circular cryptic plasmid named pPAGA 2734 bp was isolated from Pantoea agglomeransstrain EGE6 an endophytic bacterial isolate from eucalyptus. A cryptic plasmid pLU4 was isolated and sequenced from Lactobacillus reuteri strain LU4 a probiotic strain isolated from human feces in Korea. Cryptic plasmid in Oxford Dictionary of Biochemistry and Molecular Biology. Citreum C4 was characterized and its replication region was subsequently determined. The majority of these were unusually small cryptic plasmids SCPs. A new shuttle vector pUCC3E1 was constructed based on pCC3.
Cryptic plasmids are essentially in a commensal relationship with its host - the plasmid benefits by utilizing its reproductive machinery but the host gains no benefit.
An electroporation method for magnetic bacteria that uses the cryptic plasmid was also developed. The majority of these were unusually small cryptic plasmids SCPs. Isolates possess this cryptic plasmid 31. The cryptic plasmid from AN12 was isolated using a modified Qiagen plasmid purification protocol. Moreover LAB hosts for this vector were developed using kimchi LAB. The regions containing rep and mob are separated by 140-bp inverted repeat sequences and display a difference in G C content from one another.
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Placement of dimensions on a. A 1435-bp SacI-BclI fragment. The total probable coding capacity of pJD1 was determined from the complete nucleotide sequence by using computational probes to identify open reading frames with similar codon usage and by screening for the presence of ribosomal binding sites before the start. A circular cryptic plasmid named pPAGA 2734 bp was isolated from Pantoea agglomeransstrain EGE6 an endophytic bacterial isolate from eucalyptus. Inserted DNA in λ gt 11 can be expressed as.
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SCPs were phenotypically barren and the smallest of these pKL1 contained. Difficile but there is a lack of recent work examining the prevalence and heterogeneity of plasmids in this diverse bacterial species. Cryptic plasmids are essentially in a commensal relationship with its host - the plasmid benefits by utilizing its reproductive machinery but the host gains no benefit. Cryptic plasmid in Oxford Dictionary of Biochemistry and Molecular Biology. A cryptic plasmid pSM429 isolated from psychrophilic Pseudoalteromonas sp.
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Moreover LAB hosts for this vector were developed using kimchi LAB. Placement of dimensions on a. Difficile but there is a lack of recent work examining the prevalence and heterogeneity of plasmids in this diverse bacterial species. Citreum C4 was characterized and its replication region was subsequently determined. The majority of these were unusually small cryptic plasmids SCPs.
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A survey of clinical and historical isolates of C. Sequence analysis revealed that the plasmid has a GC content of 51 and contains four potential ORFs 238A 250B 131C and 129D amino acids in length without homology to known proteins. This plasmid also has the ability to delete a specific segment of itself 4 6. Isolates possess this cryptic plasmid 31. BSi20429 from the Arctic sea ice was sequenced and characterized.
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The naturally occurring cryptic plasmid pJD1 of Neisseria gonorrhoeae is 4207 base pairs long and is found in about 96 of gonococcal strains. One open reading frame RepMM similar to the rep gene of the rolling-circle replicon RCR group VIII has been allocated in the replication region where a putative double-strand origin dso and single-strand origin sso could also be. Revealed a 2725-base pair bp cryptic plasmid comprising two open reading frames of considerable length which were designated rep and mob. A new shuttle vector pUCC3E1 was constructed based on pCC3. The cryptic plasmid from AN12 was isolated using a modified Qiagen plasmid purification protocol.
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