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Haloferax Mediterranei. Haloferax mediterranei is an extremely halophilic organism that belongs to the family Halobacteriaceae in the domain Archaea. Haloferax mediterranei strain R-4 T was isolated from a saltern pond in Spain by enrichment in a defined medium with 200 g L 1 NaCl glucose as carbon source and NH 4 as nitrogen source. In this study growth kinetics and PHBV synthesis were characterised under nitrogen-excess and nitrogen-limiting conditions in. Haloferax mediterranei R4 belongs to the group of halophilic archaea one of the predominant microbial populations in hypersaline environments.
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Mediterranei of the Mediterranean Sea from L. In this work we found that deletion of the halocin H4-encoding gene halH4 blocks the DNA uptake in Hfx. Haloferax mediterranei a family member of halophilic archaea is well known for its fermentative production of poly-β-hydroxyalkanoates PHAs. PHAs are natural polymers that exhibit great potential in a wide range of applications such as a good alternative to petroleum-based plastics and the biocompatible material. Dotted line20 NaCl broken line 10 NaCl solid line5 NaCl Wavelength nm Absorbance 388. Mediterranei grows faster than most other members of Halobacteriaceae and exhibits remarkable metabolic efficiency and genome stability at.
And 23S ribosomal RNA gene partial sequence.
In these ecosystems the low availability of oxygen pushes the microbial inhabitants toward anaerobic pathways and the presence of N-oxyanions favor denitrification. TRNA-Ala gene complete sequence. Sequence accession description Seq. PHAs are natural polymers that exhibit great potential in a wide range of applications such as a good alternative to petroleum-based plastics and the biocompatible material. This study shows that halolysin R4 from Haloferax mediterranei provides its host antagonistic and defensive activities against other haloarchaea which expands our knowledge on the traditional function of haloarchaeal extracellular proteases. Haloferax mediterranei strain R-4 T was isolated from a saltern pond in Spain by enrichment in a defined medium with 200 g L 1 NaCl glucose as carbon source and NH 4 as nitrogen source.
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Accession number Sequence length bp Sequence database Associated NCBI tax ID Ref. Haloferax mediterranei a family member of halophilic archaea is well known for its fermentative production of poly-β-hydroxyalkanoates PHAs. Haloferax mediterranei R4 belongs to the group of halophilic archaea one of the predominant microbial populations in hypersaline environments. It is the first study on the function of halocins in natural environments substantially contributing to new knowledge in this domain. This strain was firstly isolated from salt evaporation ponds near Alicante Spain 1 2 and used for several studies over the last decades 3 4 5 6.
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In addition the polysaccharide has a wide temperature range and pH tolerance which accounts for its wide industrial applications Venugopal 2011. Halocin H4 HalH4 secreted by Haloferax mediterranei has always been taken as a typical antimicrobial protein. It has been tailored to synthesize polymeric materials that can be utilized for various applications. Mediterranei by changing the cell surface ultrastructure. Haloferax mediterranei is so far one of the best-studied PHA producers from the Halobacteriaceae family and it produced 486 wt PHB Don et al 2006.
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