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Human Macrophage Cell Line. A human monocyte-derived macrophage THP-1 cell line was obtained from ATCC Manassas VA and maintained as recommended by the supplier. We have evaluated the human macrophage Mφ cell line U937 as an in vitro model for human leucocyte antigen HLA class Irestricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells. In this study we characterise a human macrophage cell line derived from THP-1 cells and compare its phenotype to the THP-1 cells. Remove cells from liquid nitrogen cryostorage and thaw frozen cells rapidly 1 minute in a 37 C water bath.
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MH-S cells are a continuous cell line of murine alveolar macrophages which were established after transformation of cells obtained by bronchoalveolar lavage from. Liu M Wu M. Daigneault M et al. Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules. After 3 days the foils were gently. THP-1 cells were differentiated with the addition of PMA to their media and were cultured on steel foils for 3 days.
Transfer cell suspension to a 15 mL falcon.
THP-1 cells were differentiated with the addition of PMA to their media and were cultured on steel foils for 3 days. Do not submerge to avoid decontamination. A human monocyte-derived macrophage THP-1 cell line was obtained from ATCC Manassas VA and maintained as recommended by the supplier. U937 Page 4 of 5 APPENDIX 1 Thawing cells 1. In this study we characterise a human macrophage cell line derived from THP-1 cells and compare its phenotype to the THP-1 cells. Daigneault M et al.
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Exp Hema 208974-979 1992. Significant upregulation or downregulation in the relative gene expression levels compared to the un-differentiated cell of monocyte-like cells lines and human macrophages upon activation by either 20 nM PMA for 24 h for the cell lines and 10 ngmL GM-CSF for 6 days with or without polarization using 10 ngmL LPS and 20 ngmL IFNγ. Do not submerge to avoid decontamination. Introduction of human CSF-l cDNA into these cells eliminated their growth factor dependence but these cells were not transformed and unable to grow as tumors Roussel et al 1988. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester.
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Infection Mutagenesis High Content Screening Fluorescence Microscopy. Cell Culture Derivative Assay. These cell lines were analyzed for expression of a panel of hematopoietic. THP-1 cells were differentiated with the addition of PMA to their media and were cultured on steel foils for 3 days. Recently Sundström and Nilsson 5 reported the establishment of a human histiocytic lymphoma cell line U937 with macrophage characteristics.
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Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules. Recently Sundström and Nilsson 5 reported the establishment of a human histiocytic lymphoma cell line U937 with macrophage characteristics. Infection Mutagenesis High Content Screening Fluorescence Microscopy. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 SV4056 this technique has not in general been useful in producing human cell lines presumably. Exp Hema 208974-979 1992.
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Cell Culture Derivative Assay. These cell lines were analyzed for expression of a panel of hematopoietic. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 SV4056 this technique has not in general been useful in producing human cell lines presumably. We have evaluated the human macrophage Mφ cell line U937 as an in vitro model for human leucocyte antigen HLA class Irestricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells. Start directly from freshly isolated mononuclear cells without the need for an additional purification step with our Monocyte Attachment Medium.
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Liu M Wu M. Start directly from freshly isolated mononuclear cells without the need for an additional purification step with our Monocyte Attachment Medium. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester. Introduction of human CSF-l cDNA into these cells eliminated their growth factor dependence but these cells were not transformed and unable to grow as tumors Roussel et al 1988. The human macrophage-like cell line U937 ATCC CRL-15932 growth medium RPMI 1640 10 FCS purchased from PAA Pasching Austria was used to investigate the intracellular multiplication of Francisella strains.
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Daigneault M et al. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. Kohro T et al. When cells of human macrophage-like cell line U937 are cultured in the presence of medium conditioned by mixed lymphocyte culture MLC PHA- or Con A-stimulated lymphocytes they demonstrate morphologic and functional characteristics of stimulation. The cells become larger their surface more villou.
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Transfer cell suspension to a 15 mL falcon. Recently Sundström and Nilsson 5 reported the establishment of a human histiocytic lymphoma cell line U937 with macrophage characteristics. Exp Hema 208974-979 1992. The human macrophage-like cell line U937 ATCC CRL-15932 growth medium RPMI 1640 10 FCS purchased from PAA Pasching Austria was used to investigate the intracellular multiplication of Francisella strains. Cells being human cells is an important fact as human cell line-derived macrophages could represent the molecular mechanism of human macrophages more precisely than mouse cells which are used for many MAF assays 11 since human cells share the same gene set or genome set.
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Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules. Three human monocytic cell lines U-937 THP-1 and Mono Mac 6 have because of their morphology and staining properties been classed as cell lines frozen in a window of the monocyte differentiation lineage corresponding to monoblasts andor immature monocytes. The identification of markers of macrophage differentiation in PMA-stimulated THP-1 cells and monocyte-derived macrophages. They were isolated from the histiocytic lymphoma of a 37-year-old male patient and are used to study the behaviour and differentiation of monocytes. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 SV4056 this technique has not in general been useful in producing human cell lines presumably.
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Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester. Significant upregulation or downregulation in the relative gene expression levels compared to the un-differentiated cell of monocyte-like cells lines and human macrophages upon activation by either 20 nM PMA for 24 h for the cell lines and 10 ngmL GM-CSF for 6 days with or without polarization using 10 ngmL LPS and 20 ngmL IFNγ. Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules. Recently Sundström and Nilsson 5 reported the establishment of a human histiocytic lymphoma cell line U937 with macrophage characteristics. Culture of the Human Monocyte-Macrophage Cell Line.
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Transfer cell suspension to a 15 mL falcon. THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line Daisy using microscopy flow cytometry phagocytosis assays antigen binding assays and gene microarrays. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester. Kohro T et al. Remove cells from liquid nitrogen cryostorage and thaw frozen cells rapidly 1 minute in a 37 C water bath.
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A human monocyte-derived macrophage THP-1 cell line was obtained from ATCC Manassas VA and maintained as recommended by the supplier. When cells of human macrophage-like cell line U937 are cultured in the presence of medium conditioned by mixed lymphocyte culture MLC PHA- or Con A-stimulated lymphocytes they demonstrate morphologic and functional characteristics of stimulation. Culture of the Human Monocyte-Macrophage Cell Line. Start directly from freshly isolated mononuclear cells without the need for an additional purification step with our Monocyte Attachment Medium. Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules.
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Liu M Wu M. After 3 days the foils were gently. Macrophage Cell Culture Complete macrophage research portfolio including cryopreserved monocyte-derived human M1M2 macrophages xeno-free in vitro generation media and additional reagents. The human macrophage-like cell line U937 ATCC CRL-15932 growth medium RPMI 1640 10 FCS purchased from PAA Pasching Austria was used to investigate the intracellular multiplication of Francisella strains. THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line Daisy using microscopy flow cytometry phagocytosis assays antigen binding assays and gene microarrays.
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Introduction of human CSF-l cDNA into these cells eliminated their growth factor dependence but these cells were not transformed and unable to grow as tumors Roussel et al 1988. THP-1 cells with and without mitogenic stimulation were compared to the newly derived macrophage-like cell line Daisy using microscopy flow cytometry phagocytosis assays antigen binding assays and gene microarrays. We have evaluated the human macrophage Mφ cell line U937 as an in vitro model for human leucocyte antigen HLA class Irestricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells. Culture of the Human Monocyte-Macrophage Cell Line. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 SV4056 this technique has not in general been useful in producing human cell lines presumably.
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MH-S cells are a continuous cell line of murine alveolar macrophages which were established after transformation of cells obtained by bronchoalveolar lavage from. The human macrophage-like cell line U937 ATCC CRL-15932 growth medium RPMI 1640 10 FCS purchased from PAA Pasching Austria was used to investigate the intracellular multiplication of Francisella strains. Significant upregulation or downregulation in the relative gene expression levels compared to the un-differentiated cell of monocyte-like cells lines and human macrophages upon activation by either 20 nM PMA for 24 h for the cell lines and 10 ngmL GM-CSF for 6 days with or without polarization using 10 ngmL LPS and 20 ngmL IFNγ. Liu M Wu M. U937- and THP-1 cell line-derived macrophages could offer.
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When cells of human macrophage-like cell line U937 are cultured in the presence of medium conditioned by mixed lymphocyte culture MLC PHA- or Con A-stimulated lymphocytes they demonstrate morphologic and functional characteristics of stimulation. Although murine macrophage-like lines have been obtained by transformation with simian virus 40 SV4056 this technique has not in general been useful in producing human cell lines presumably. Evaluated the human macrophage Mw cell line U937 as an in vitro model for human leucocyte antigen HLA class I-restricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells in the absence of detectable HLA class II or CD1 molecules. Exp Hema 208974-979 1992. When cells of human macrophage-like cell line U937 are cultured in the presence of medium conditioned by mixed lymphocyte culture MLC PHA- or Con A-stimulated lymphocytes they demonstrate morphologic and functional characteristics of stimulation.
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Daigneault M et al. The SV40-immortalized mouse macrophage cell line BACl2F5 is dependent on CSF-l for survival in culture. Daigneault M et al. Recently Sundström and Nilsson 5 reported the establishment of a human histiocytic lymphoma cell line U937 with macrophage characteristics. Culture of the Human Monocyte-Macrophage Cell Line.
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They were isolated from the histiocytic lymphoma of a 37-year-old male patient and are used to study the behaviour and differentiation of monocytes. A human monocyte-derived macrophage THP-1 cell line was obtained from ATCC Manassas VA and maintained as recommended by the supplier. Infection Mutagenesis High Content Screening Fluorescence Microscopy. Liu M Wu M. Induction of human monocyte cell line U937 differentiation and CSF-1 production by phorbol ester.
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Culture of the Human Monocyte-Macrophage Cell Line. We have evaluated the human macrophage Mφ cell line U937 as an in vitro model for human leucocyte antigen HLA class Irestricted presentation of mycobacterial antigens as HLA class I is constitutively expressed at high levels by U937 cells. Infection Mutagenesis High Content Screening Fluorescence Microscopy. Culture of the Human Monocyte-Macrophage Cell Line. THP-1 cells were differentiated with the addition of PMA to their media and were cultured on steel foils for 3 days.
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