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Iodoacetamide. Molecular Weight 18496. After reduction the sulfhydryls are usually reacted with iodoacetamide IAM iodoacetic acid IAA or another electrophile to prevent reformation of disulfide linkages in a random manner. Iodoacetamide BioUltra CAS Number 144-48-9. Molecular Weight 18496.
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The compound with a radioactive or fluorescent tag is used label reduced thiols. Monoisotopic mass 184933746 Da. PubChem Substance ID 24278486. Molecular Formula C 2 H 4 INO. Iodoacetamide can react with low molecular weight thiol. Glycosyl iodoacetamide found useful applications in the synthesis of N-linked glycoprotein mimics wherein the cysteine residue in peptide reacts selectivity in solution with the modified glycan.
Iodoacetamide is routinely used in proteomics to distinguish protein oxidation states.
Monoisotopic mass 184933746 Da. Articles of Iodoacetamide are included as well. PubChem Substance ID 24278486. Benzophenone-4-iodoacetamide C15H12INO2 CID 131289 - structure chemical names physical and chemical properties classification patents literature biological. Alkylation with iodoacetamide after cystine reduction results in the covalent addition of a carbamidomethyl group 5707 Da and prevents the formation of disulfide bonds. Iodoacetamide based isotope-coded affinity tag ICAT reagents can be used to label free cysteine thiols then mass spectrometry can be used to quantitate the relative labelling of free thiols before 12C and after 13C treatment to either oxidise or reduce proteins 52.
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Benzophenone-4-iodoacetamide C15H12INO2 CID 131289 - structure chemical names physical and chemical properties classification patents literature biological. Glycosyl iodoacetamide found useful applications in the synthesis of N-linked glycoprotein mimics wherein the cysteine residue in peptide reacts selectivity in solution with the modified glycan. Alkylating reagent for cysteine and histidine residues in proteins. Molecular Weight 18496. Iodoacetamide BioUltra CAS Number 144-48-9.
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