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Near Isogenic Lines. A high seed protein line HiPro with the high seed. Near-isogenic lines NILs are strains which genetic makeups are identical except for few specific locations or genetic loci Muehlbauer et al 1988. Near isogenic lines are generated by a process of repeated backcrossing with selection for the desired. In the BC8 generation the 32 kb infiltrated the specific marker scanning located gene fragment.
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The development of near isogenic lines NILs. Analysis of near-isogenic lines NILs is an essential approach in the characterization of target loci in quantitative genetic studies Tuinstra et al. Interestingly through the analyses of recombinant inbred lines RILs developed from a cross between Tachinagaha and Iyodaizu quantitative trait loci QTLs for root development under hypoxia at the seedling stage of soybean were detected on chromosome 12 Chr12We investigated whether the candidate QTL region for root development is involved in seed waterlogging tolerance by using a near. The use of NILs minimizes the effects caused by different genetic backgrounds to avoid epistatic gene interactions that may occur in outbred strains Zhu et al 2015. Near isogenic lines are generated by a process of repeated backcrossing with selection for the desired. Near-isogenic lines NILs are strains which genetic makeups are identical except for few specific locations or genetic loci Muehlbauer et al 1988.
Frequencies of self-incompatibility alleles in a natural population of 24 Paran I Kesseli R and Michelmore R Identification of restriction Sinapis arvensis L.
The details of near-isogenic lines development are described in the work of Miura et al. Multiple pairs of isolines can offer a common genetic background to assess the phenotype conditioned by a genomic locus. Near Isogenic Lines NILs Near isogenic lines are frequently the product of plant breeding programs where the objective has been the introgression of a desirable character or trait gene from a donor parent into an otherwise agronomically acceptable cultivar recurrent parent. NILs are fixed homozygous lines that share the same genetic background from a recurrent parent with a single introgression region from a donor parent. Nineteen genomic regions identiWed by restriction fragment length polymorphism loci and chosen to rep-resent portions of all ten maize chromosomes were. Theor Appl Genet 2 Stevens JP and Kay QON The number dominance relationships and 110640648 2005.
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Five F 7 RILs from the mapping population were chosen as founders of HIFs on the basis of being heterozygote for the 4A-1 QTL region Fig. Nonetheless depending on the available resources and the purpose of the lines to be generated several choices can be made to guide the design of such inbred. Near-isogenic lines have otherwise identical genetic backgrounds except at one or a few genetic loci and have been used intensively for detailed mapping and characterization of individual loci. In this study we sought to prepare genetically homogeneous plant materials harboring Rpp s for which we generated near-isogenic lines NILs of Rpp s. Starch preparationStarches from near-isogenic wheat types were extracted as described in the paper of Mangalika et al.
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For example a Plugging plg-1 e2001III strain in the N2 background was built in. Near-isogenic lines have otherwise identical genetic backgrounds except at one or a few genetic loci and have been used intensively for detailed mapping and characterization of individual loci. Starch preparationStarches from near-isogenic wheat types were extracted as described in the paper of Mangalika et al. Tingdong Fu was used to establish a near-isogenic line NIL through 13 generations of backcrossing Source. Generation of multiple near-isogenic lines and RNA-seq analysis We generated multiple NILs segregating for the 4A-1 QTL following a method based on HIFs analysis 8.
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Two near-isogenic lines were developed Nichols et al 2006 that contrast in both genetic information at the LG I seed protein QTL and in corresponding seed protein levels. NILs are a pair of plants possessing almost the same genetic background except at one single gene or locus. Near-isogenic lines NILs are strains which genetic makeups are identical except for few specific locations or genetic loci Muehlbauer et al 1988. Near-isogenic lines NILs and introgression lines are useful for QTL discovery and validation and as a resource to initiate positional cloning projects. Near-isogenic lines NILs are tools to dissect quantitative characters and identify some of their components as Mendelian traits.
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