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Phluorin. Christian Rosenmunds lab contains the insert lyso-pHluorin and is published in Nat Neurosci. Optical indicators provide a powerful means to reveal such distributed activity particularly when protein-based and encodable in DNA. RRIDAddgene_32001 For your References section. Here we have turned pHluorin inside-out and show that cytoplasmic fusions of pHluorin are effective quantita-tive reporters for endocytosis and multivesicular.
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Some of such GFP derivatives have been termed pHluorins with classes of pHluorins including ecliptic pHluorin ie pH-dependent change in the intensity of emission at a single excitation wavelength and ratiometric pHluorin ie pH-dependent change in the ratio of the intensity of emission at a shorter excitation wavelength vs. Neurotransmitter release can also be examined using the genetically encoded synaptic transmission reporter synapto-pHluorin which uses pH-sensitive mutants of GFP. Red-shifted ecliptic pHluorin that is excited strongly by blue light at neutral pH but minimally fluorescent at acidic pH. Amiloride inhibits macropinocytosis by lowering submembranous pH and preventing Rac1 and Cdc42 signaling. Christian Rosenmunds lab contains the insert lyso-pHluorin and is published in Nat Neurosci. Here we have turned pHluorin inside-out and show that cytoplasmic fusions of pHluorin are effective quantita-tive reporters for endocytosis and multivesicular.
RRIDAddgene_32001 For your References section.
PHTomato has a p K a of 78 so it is practically nonfluorescent below pH 63. Encodable probes can be introduced into cells tissues or tra. RRIDAddgene_32001 For your References section. In neural systems information is often carried by ensembles of cells rather than by individual units. However the docking step cannot be visualized using this FP since the. An enhanced ratiometric pH-sensitive green florescent protein.
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PHTomato has a p K a of 78 so it is practically nonfluorescent below pH 63. Here we have turned pHluorin inside-out and show that cytoplasmic fusions of pHluorin are effective quantita-tive reporters for endocytosis and multivesicular. Recently a pH sensitive red fluorescent protein pHTomato has been developed as an alternative to pHluorins for monitoring exocytosis 12. PHTomato has a p K a of 78 so it is practically nonfluorescent below pH 63. An enhanced ratiometric pH-sensitive green florescent protein.
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Superecliptic pHluorin SEP a green pH-sensitive FP has been widely used for imaging single-vesicle exocytosis. Recently a pH sensitive red fluorescent protein pHTomato has been developed as an alternative to pHluorins for monitoring exocytosis 12. PHluorin ratiometric is a multistate long stokes shift fluorescent protein published in 1998 derived from Aequorea victoria. Red-shifted ecliptic pHluorin that is excited strongly by blue light at neutral pH but minimally fluorescent at acidic pH. Here we have turned pHluorin inside-out and show that cytoplasmic fusions of pHluorin are effective quantita-tive reporters for endocytosis and multivesicular.
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