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Reca1. The recA1 mutation is a single point mutation that replaces glycine 160 of the recA polypeptide with an aspartic acid residue in order to disable the activity of the recombinases and inactivate homologous recombination. The mutations that the DH5-Alpha strain has are. The gene coding for the RECA1 protein derived from Arabidopsis thaliana of the present invention regulates gene expression associated with biotic. Gene recA1 a gene involved in recombination.
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Borders of expressed constructs are indicated by dotted lines. The endothelium is a single layer of cells lining various organs and cavities of the body especially the blood vessels heart and lymphatic vesselsEndothelial cells line the entire circulatory system from the heart to the smallest capillary. G to A for Nuc. The XL1-Blue strain provides a host for optimal propagation of both plasmid and lambda phage vectors. The present invention relates to a production method for a genetically modified plant having enhanced immune capacity with respect to pathogenic microorganisms using the RECA1 gene derived from Arabidopsis thaliana and relates to a plant therefrom. RecA1 is deficient in all known function of the RecA gene specifically in ATPase activity binding with DNA in the presence of ATP and changing conformation in the presence of ATP and repressor cleavage.
The recA1 mutation is a single point mutation that replaces glycine 160 of the recA polypeptide with an aspartic acid residue in order to disable the activity of the recombinases and inactivate homologous recombination.
RecA1 is an inactivated form of RecA. And I know that DH5a JM109 Xl-1 blue etc. Gene recA1 a gene involved in recombination. Also known as RECA1_MYXXA recA1. G to A for Nuc. Each of these strains also carries the endA1 mutation that inactivates a nuclease that might.
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Zoom In Zoom Out Reset. A recombination deficient mutation of E. These mutations correspond to the distinct characteristics that make the DH5-Alpha strain excel in laboratory cloning procedures 2. Each of these strains also carries the endA1 mutation that inactivates a nuclease that might. Genotype fhuA2 ΔargF-lacZU169 phoA glnV44 Φ80 ΔlacZM15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17 Reagents Supplied.
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And I know that DH5a JM109 Xl-1 blue etc. Base Pair Substitution Mutation of Gene. RecA1 is deficient in all known function of the RecA gene specifically in ATPase activity binding with DNA in the presence of ATP and changing conformation in the presence of ATP and repressor cleavage. Zoom In Zoom Out Reset. In addition to the G-patch motif NKRF has three RNA binding domains RBD and another nucleic acid binding domain termed R3H.
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