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Vglut2. Vesicular glutamate transporters VGluTs are proton-dependent carriers responsible for. Specificity Specific for VGLUT 2. Of the three known transporters VGLUT1 and VGLUT2 are expressed prominently in the adult brain but during the first two weeks of postnatal development VGLUT2 expression predominates. Vesicular glutamate transporter 2 UniProt Q9JI12.
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Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells PubMed11551935 PubMed11698619 PubMed11698620 PubMed11502256 PubMed17046815 PubMed18080752. Specificity Specific for VGLUT 2. Three transporters VGLUT1-VGLUT3 are expressed in the mammalian CNS with partial overlapping expression patterns and VGLUT2 is the most. D Expression of the VGLUT2 protein 56 kDa in brain derived from 185-d-old VGLUT2 embryos lane 1 was absent in the VGLUT2 embryos whereas intermediate levels were observed in VGLUT2 embryos lane 2 as determined by Western blot analysis. Other species not tested yet. Ad Wide species reactivity highly cited and reviewed VGLUT2 antibodies.
Vglut2 flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 sodium-dependant inorganic phosphate cotransporter member 6 Slc17a6 or Vglut2 gene.
The ability of 5-HT cholinergic and dopamine DA neurons to release glutamate at synapses has been demonstrated in vitro 79. A targeting vector constructed from a BAC clone containing the Slc17a6 Vglut2 gene was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc17a6 locus just downstream of the endogenous stop codon. The highly homologous protein VGLUT2 also initially characterized as a phosphate transporter and named differentiation-associated Na -dependent inorganic phosphate transporter DNPi has a nearly complementary pattern of expression with one of the two proteins present in all established glutamatergic neurons. Other species not tested yet. Ad Wide species reactivity highly cited and reviewed VGLUT2 antibodies. Also known as Differentiation-associate d BNPI Differentiation-associate d Na-dependent inorganic phosphate cotransporte Solute carrier family 17 member 6 VGluT2 is encoded by the Slc17a6 also known as Dnpi Vglut2 gene Gene ID 84487 in rat species.
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VGLUT2 is transiently expressed in most DA neurons during development in the mouse. Docking and homology modeling explain the inhibition of VGLUT2. D Expression of the VGLUT2 protein 56 kDa in brain derived from 185-d-old VGLUT2 embryos lane 1 was absent in the VGLUT2 embryos whereas intermediate levels were observed in VGLUT2 embryos lane 2 as determined by Western blot analysis. In general VGLUT1 predominates in the neocortex and cerebellar cortex whereas VGLUT2. VGluT2 is the only vesicular glutamate transporter expressed in transgenic ventral tegmental areasubstantia nigra dopamine neurons in vivo.
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Of the three currently known VGLUTs VGLUT2 is the member predominantly expressed in the more phylogenetically primitive areas of the brain including the hypothalamus and brainstem. A subset of adult ventral tegmental area dopamine DA neurons expresses vesicular glutamate transporter 2 VGluT2 and releases glutamate as a second neurotransmitter in the striatum while only few adult substantia nigra DA neurons have this capacity. Cre is expressed from the Slc17a6 gene promoter. Vesicular glutamate transporter 2 UniProt Q9JI12. Human rat mouse chicken.
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The highly homologous protein VGLUT2 also initially characterized as a phosphate transporter and named differentiation-associated Na -dependent inorganic phosphate transporter DNPi has a nearly complementary pattern of expression with one of the two proteins present in all established glutamatergic neurons. Vglut2 flox mutant mice possess loxP sites flanking exon 2 of the solute carrier family 17 sodium-dependant inorganic phosphate cotransporter member 6 Slc17a6 or Vglut2 gene. VGLUT1 and 2 are also expressed in some cholingergic and GABAergic circuits where co-released neurotransmitters may engage. Involved in the regulation of retinal hyaloid vessel regression during postnatal development By. Other species not tested yet.
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A subset of adult ventral tegmental area dopamine DA neurons expresses vesicular glutamate transporter 2 VGluT2 and releases glutamate as a second neurotransmitter in the striatum while only few adult substantia nigra DA neurons have this capacity. Beta Tubulin 3 Tuj1 stained by beta Tubulin 3 Tuj1 antibody GT1338 GTX631831 diluted at 1500. Human rat mouse chicken. Quantal neurotransmitter release at excitatory synapses depends on glutamate import into synaptic vesicles by vesicular glutamate transporters VGLUTs. VGluT2 is the only vesicular glutamate transporter expressed in transgenic ventral tegmental areasubstantia nigra dopamine neurons in vivo.
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Involved in the regulation of retinal hyaloid vessel regression during postnatal development By. Beta Tubulin 3 Tuj1 stained by beta Tubulin 3 Tuj1 antibody GT1338 GTX631831 diluted at 1500. A targeting vector constructed from a BAC clone containing the Slc17a6 Vglut2 gene was utilized to insert an internal ribosomal entry site followed by the cre recombinase sequence into the Slc17a6 locus just downstream of the endogenous stop codon. Glutamate dissipates ψ and increases pH facilitating vesicular storage of dopamine. The ability of 5-HT cholinergic and dopamine DA neurons to release glutamate at synapses has been demonstrated in vitro 79.
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